The International Atomic Energy Agency (IAEA) had warned Saturday that the "disproportionate and unprecedented" move to bar inspectors would seriously hamper its work in Iran.
Iranian media and a Western diplomat said the decision concerned eight inspectors, all from France and Germany.
"Iran must immediately reverse these inspector de-designations and fully cooperate with the Agency to enable them to provide assurances that Iran's nuclear programme is exclusively peaceful," the permanent representatives to the IAEA of France, Germany, the United Kingdom, and the United States said in a joint statement.
The four countries "will continue to stand in strong support of the IAEA and the international safeguards verification regime on which the world's security relies," it added.
IAEA head Rafael Grossi on Monday also urged Iran to reconsider its decision, warning that Iran's failure to cooperate would carry severe consequences.
"If they do not cooperate with the IAEA, they will not get what they want: the assurances they want to see, the confirmation they want to see, the approval of the international community," Grossi told AFP in an interview.
Brussels, which acts as coordinator for the 2015 nuclear deal between Tehran and world powers known as the JCPOA, on Sunday said it was "highly concerned" by the development.
In 2015, major world powers reached the accord with Iran under which Tehran would curb its nuclear programme in exchange for relief from crippling economic sanctions.
But that started to unravel in 2018 when then-US president Donald Trump withdrew from the deal and reimposed sanctions.
Tehran in turn stepped up its nuclear programme, while continuing to deny that it harbours ambitions of developing nuclear weapons capability.
Efforts to revive the deal have been fruitless so far.
US-Iran ties since Trump quit nuclear deal
Paris (AFP) Sept 18, 2023 -
Following are key dates in the storied relationship between the United States and its arch-rival Iran since Washington pulled out of a landmark nuclear pact with Tehran in 2018.
- 2018: nuclear deal walkout -
After years of mounting concern over Iran's nuclear enrichment programme, world powers reach a deal with Tehran on sanctions relief in exchange for guarantees it will not build an atomic bomb.
Three years later, then president Donald Trump pulls the United States out of the deal and reapplies the financial pain.
In April 2019, Washington designates Iran's elite military force, the Islamic Revolutionary Guard Corps, a terrorist organisation.
Tehran, meanwhile, begins rolling back some of the commitments it made under the nuclear deal on uranium enrichment.
- 2019: Gulf tensions -
In, 2019 tension mounts after attacks on oil tankers in the Gulf and on oil facilities in Saudi Arabia, for which the United States blames Iran.
In June Trump authorises -- and then calls off at the last moment -- a military strike after Iran shoots down an American drone.
- 2019: Soleimani killed -
In December, protesters storm the American embassy in Baghdad following deadly US air strikes on Iran-backed militiamen in Iraq and Syria.
On January 3, 2020, a US drone strike on Baghdad's international airport kills top Iranian general Qasem Soleimani and a pro-Iran Iraqi commander.
Tensions remain high throughout Trump's presidency, which ends in January 2021.
- 2022: nuclear deal 'dead' -
In April 2021 talks aimed at saving the international nuclear deal with Iran begin in Vienna.
The United States is indirectly invited for the first time since Joe Biden, a Democrat, was elected president.
The European Union, which is coordinating the talks, submits a compromise text in August 2022 but the negotiations fail.
At a campaign rally in November 2022, Biden says the deal is "dead" in all but name.
- September 2023: prisoner swap -
The two adversaries make more progress in talks over a prisoner swap, reaching an accord in August to release five Americans for five Iranians plus the unblocking of $6 billion in frozen Iranian assets.
It is the first deal with Iran's clerical leadership that Biden has reached in his two and a half years as president.
On September 18, five freed US detainees fly out of Tehran to Qatar and two of the five freed Iranian prisoners arrive in the Gulf state from the United States.
The timing of the exchange angers rights groups, coming two days after the first anniversary of the death in Iran of Mahsa Amini, a young Kurdish Iranian woman arrested for allegedly violating the country's strict headscarf law.
Despite the prisoner swap, Washington announces sanctions against dozens of Iranian officials and three media groups, as well as against Iran's intelligence ministry and former president Mahmoud Ahmadinejad.
"We will continue to impose costs on Iran for their provocative actions in the region," Biden says.
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