One of the startling discoveries about life on Earth in the past 25 years is that it can - and does - flourish beneath the ocean floor, in the planet's dark, dense, rocky crust. The only way to get there is by drilling through meters of sediment until you hit rock, so information on this ubiquitous but buried marine biosphere is still scarce.

Now, a team led by MBL Associate Scientist Julie Huber has added new details to our understanding of the nature of life way down under. In a recent paper, she and her colleagues offer the first description of an active microbial community buried in cold oceanic crust at North Pond, an isolated sediment pond on the western flank of the Mid-Atlantic Ridge.

The oceanic crust is anything but static: Seawater runs through its rocky crevices, creating a dynamic aquifer through which the entire volume of the ocean circulates every 200,000 years. Huber's team discovered that the microbial community in North Pond samples was oxygenated, heterogeneous, and markedly distinct from that found in ocean bottom seawater.

"In many cases, we found the same general group [of bacteria] in the aquifer and in bottom seawater, but different species within that group," Huber said. That means distinct differences in potential microbial activity between the two sites, such as more carbon fixation in the aquifer.

This is the first paper to describe the subseafloor microbial community in a cold crustal aquifer site. Prior work has focused on the hot, volcanic fluids at mid-ocean ridges and the subseafloor microbes that survive there.

"The cold crustal aquifer is a different environment that is also globally important not just in terms of life, but biogeochemical cycling," Huber says. "We are only starting to discover how things proceed there."

The samples were obtained from a subseafloor observatory installed at North Pond by the Integrated Ocean Drilling Program in 2011. Combining genomic technologies with geochemical measurements, Huber's team examined crustal fluid samples retrieved in 2012 from 50 to 250 meters beneath the seafloor, under h 4.5 kilometers of seawater.

They are working on a time series to detect if and how the microbial community and fluid chemistry changes; they are currently analyzing 2014 samples and will collect more in 2017.

Meyer, Julie L, Ulrike Jaekel, Benjamin J. Tully, Brian T. Glazer, C. Geoffrey Wheat, Huei-Ting Lin, Chih-Chiang Hsieh, Samuel M. Hulme, James P. Cowen, Peter R. Girguis, Julie A. Huber (2016) A distinct and active bacterial community in cold oxygenated fluids circulating beneath the western flank of the Mid-Atlantic ridge. Scientific Reports 6, doi:10.1038/srep22541